ヤマモト　マキ   YAMAMOTO Maki   山本 真紀    所属   専修大学  商学部    職種   教授
言語種別	日本語
発行・発表の年月	2007/01
形態種別	研究論文（学術雑誌）
標題	Protoplast formation of the Coccolithophorid Pleurochrysis haptonemofera in Hypoosmotic K+ solution: shedding of the coccosphere and regrowth of the protoplast in normal medium
執筆形態	共著
掲載誌名	Springer New York 「Marine Biotechnology」
巻・号・頁	9(1),56-65頁
著者・共著者	Takayanagi, Hirokawa, Yamamoto, Ohki, Fujiwara, Tsuzuki
概要	Both coccolith-bearing cells (C-cells) and naked cells (N-cells) of the coccolithophorid Pleurochrysis haptonemofera can grow in salinities of more than 7‰ (about 20% of a “normal” sea water salinity [35‰]), with the highest growth rates in salinities of more than 14‰. Microscopic observations of cells suspended in 100 mM NaCl (7‰) showed that, while N-cells were swelling uniformly all over the cell surface, C-cells were bulging the plasma membrane from the hole of the coccosphere at the apical (flagellar) pole of the cell. Effects of several cations and anions on the morphological change of C-cells under hypoosmotic pressure were investigated. When 100 mM K+ was used, protoplasts were released from the coccosphere completely in almost all the cells. This phenomenon was shown with K+ most effectively. The protoplasts could grow in the fresh medium and form the first coccolith within 9 h.
DOI	10.1007/s10126-006-6058-9