イシカネ　ヒロシ   ISHIKANE Hiroshi   石金 浩史    所属   専修大学  人間科学部    専修大学大学院  文学研究科    職種   教授
言語種別	英語
発行・発表の年月	2022/11/01
形態種別	研究論文
査読	査読あり
標題	Synaptic pruning through glial synapse engulfment upon motor learning
執筆形態	共著
掲載誌名	Nature Neuroscience
掲載区分	国外
出版社・発行元	Nature Publishing Group
巻・号・頁	25(11),pp.1458-1469
著者・共著者	Morizawa, Y.M., Matsumoto, M., Nakashima, Y., Endo, N., Aida, T., Ishikane, H., Beppu, K., Moritoh, S., Inada, H., Osumi, N., Shigetomi, E., Koizumi, S., Yang, G., Hirai, H., Tanaka, K., Tanaka, K.F., Ohno, N., Fukazawa, Y., Matsui, K.
概要	Synaptic pruning is a fundamental process of neuronal circuit refinement in learning and memory. Accumulating evidence suggests that glia participates in sculpting the neuronal circuits through synapse engulfment. However, whether glial involvement in synaptic pruning has a role in memory formation remains elusive. Using newly developed phagocytosis reporter mice and three-dimensional ultrastructural characterization, we found that synaptic engulfment by cerebellar Bergmann glia (BG) frequently occurred upon cerebellum-dependent motor learning in mice. We observed increases in pre- and postsynaptic nibbling by BG along with a reduction in spine volume after learning. Pharmacological blockade of engulfment with Annexin V inhibited both the spine volume reduction and overnight improvement of motor adaptation. These results indicate that BG contribute to the refinement of the mature cerebellar cortical circuit through synaptic engulfment during motor learning.
DOI	10.1038/s41593-022-01184-5
researchmap用URL	https://researchmap.jp/ishikane/published_papers/40384627