クメ　マナブ   Manabu Kume   久米　学    所属   石巻専修大学  理工学部    職種   准教授
言語種別	英語
発行・発表の年月	2022/05/05
形態種別	研究論文（学術雑誌）
査読	査読あり
標題	Morphological and Molecular Gonadal Sex Differentiation in the Wild Japanese eel Anguilla japonica
執筆形態	未選択
掲載誌名	Cells
出版社・発行元	{MDPI} {AG}
巻・号・頁	11(9),pp.1554-1554
著者・共著者	Moemi Horiuchi,Seishi Hagihara,Manabu Kume,Daichi Chushi,Yuya Hasegawa,Hikaru Itakura,Yoh Yamashita,Shinji Adachi,Shigeho Ijiri
概要	<jats:p>Most cultured Japanese eels (Anguilla japonica) show male sex differentiation; however, natural gonadal sex differentiation has not been evaluated. In this study, this process was characterized in wild eels. Differentiated ovaries and testes were observed after the eels grew to 320 and 300 mm in total length, respectively. The youngest ovary and testis appeared at 3 and 4 years old, respectively; however, undifferentiated gonads were found up to 7 years, suggesting that sex differentiation was triggered by growth rather than aging. gsdf, amh, foxl2b and foxl3b were highly expressed in the testes, whereas figla, sox3, foxn5, zar1, and zp3 were highly expressed in the ovaries. The expression of cyp19a1a and foxl2a did not differ significantly between the testis and ovary. In the ovaries, the cyp19a1a and foxl2a levels were highest in the early stages, suggesting that their function is limited to early ovarian differentiation. The foxn5, zar1 and zp3 levels tended to increase in the later stages, suggesting that they function after the initiation of ovarian differentiation. In undifferentiated gonads, dimorphic gene expression was not observed, suggesting that the molecular sex differentiation phase is short and difficult to detect. These findings provide the first demonstration of the whole course of natural gonadal sex differentiation in eels at molecular and morphological levels.</jats:p>
DOI	10.3390/cells11091554
ISSN	2073-4409
Put Code(ORCID)	113586966