クメ　マナブ   Manabu Kume   久米　学    所属   石巻専修大学  理工学部    職種   准教授
言語種別	英語
発行・発表の年月	2020
形態種別	研究論文（学術雑誌）
査読	査読あり
標題	Evaluation of fish biodiversity in estuaries using environmental DNA metabarcoding.
執筆形態	未選択
掲載誌名	PLoS ONE
掲載区分	国外
巻・号・頁	15(10),pp.e0231127
著者・共著者	Hyojin Ahn,Manabu Kume,Yuki Terashima,Feng Ye,Satoshi Kameyama,Masaki Miya,Yoh Yamashita,Akihide Kasai
概要	Biodiversity is an important parameter for the evaluation of the extant environmental conditions. Here, we used environmental DNA (eDNA) metabarcoding to investigate fish biodiversity in five different estuaries in Japan. Water samples for eDNA were collected from river mouths and adjacent coastal areas of two estuaries with high degrees of development (the Tama and Miya Rivers) and three estuaries with relatively low degrees of development (the Aka, Takatsu, and Sendai Rivers). A total of 182 fish species across 67 families were detected. Among them, 11 species occurred in all the rivers studied. Rare fishes including endangered species were successfully detected in rich natural rivers. Biodiversity was the highest in the Sendai River and lowest in the Tama River, reflecting the degree of human development along each river. Even though nutrient concentration was low in both the Aka and Sendai Rivers, the latter exhibited greater diversity, including many tropical or subtropical species, owing to its more southern location. Species composition detected by eDNA varied among rivers, reflecting the distribution and migration of fishes. Our results are in accordance with the ecology of each fish species and environmental conditions of each river.
DOI	10.1371/journal.pone.0231127
PMID	33022692